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This is certainly especially true for immunohistochemistry by which 10% NBF has been demonstrated to get a poor fixative (Fig. two). Some issues with the usage of 10% NBF as a fixative happen to be corrected through the introduction of other experimental procedures including antigen retrieval (Shi et al. 2001) and using tiny amplicons in RT-Q-PCR (
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Enable’s speak about dehydration process, working with xylene-free protocol that includes isopropyl Liquor or IPA. For your xylene-cost-free protocols you'll likely have two methods of eighty/20 ethanol/isopropanol blend followed by three measures of absolute isopropanol. Given that Xylene is a comparatively dangerous solvent, laboratories are un